This project will develop an accurate, reliable and internationally recognised DNA based protocol to detect Karnal bunt. The methodology will be validated by laboratories in Australia and overseas and the resulting Diagnostic Protocol will be endorsed by the Sub-committee on Plant Health Diagnostic Standards and the Plant Health Committee. This practical biosecurity tool will support the Plant Health Australia National Karnal Bunt Contingency Plan.

What is the biosecurity problem?

Australia does not have Karnal bunt and is a considerable distance away from known infested areas. Quarantine is extremely important to maintain our Karnal bunt-free status. Any incursion would cause severe disruption to Australia's international wheat trade. The current diagnostic protocol involves identification of spores based on morphology followed by germination of the spores and a molecular protocol to confirm the identity. Microscopy and spore germination is slow and labour-intensive. The objective of this project is to implement an internationally recognised and cost-effective molecular assay for Tilletia indica that does not require spore germination or microscopy.

The main outputs of this project are to:

• develop an internationally recognised and cost-effective molecular assay that does not require spore germination or microscopy, and
• develop improved extraction of spores from grain and other commodities.

Who will be the end-users of this research?

The protocol will be used by biosecurity diagnosticians in federal and state government agencies and by industry, both nationally and internationally. The protocol will be made available electronically via the Diagnostic Database Web Site or as hard copy from the Office of the Chief Plant Protection Officer.